Efficacy of a New Slow Release Mosquito Larvicide

Tumaini (CRT) Inc. Efficacy of a New Slow Release Mosquito Larvicide containing Novaluron

SLOW RELEASE MOSQUITO LARVICIDE DETAILS  Larvicide contains the active ingredient novaluron and utilizes novel

formulation technology which has been patented in numerous countries  Formulation controls rate of release of novaluron into water column  increases solubility of novaluron in water from 3 ppb to 10-15 ppb

 Formulation also promotes dispersion of novaluron throughout the

water column  products will be available in several different sizes and shapes to address

different market needs  all products have same recipe and ai 1.2g/kg novaluron  Tradename for larvicide products are:

Mosquiron 0.12P and Mosquiron 0.12CRD

MOSQUIRON PRODUCT DETAILS Mosquiron 0.12P pastilles (2 sizes)

Mosquiron 0.12CRD controlled release device

MOSQUIRON PRODUCT DETAILS

Mosquiron 2010 Field Trials PURPOSE:  Assess efficacy and duration of Mosquiron products when used

under simulated field conditions and in different regions of the US and Canada

Mosquiron 2010 Field Trials Experimental Design  Needed to take into consideration the mode of action of novaluron  Novaluron is a chitin synthesis inhibitor and effects can be cumulative;

larvae can appear healthy and mortality may not be observed until adults emerge from pupal case  At high concentrations of novaluron (>5 ppb) effect on larvae can be

rapid – 24 hours  Mortality can still occur at pupal eclosion caused by incomplete

separation from pupal case – adult drowning Field Trial Locations  Canada – Portage la Prairie MB, Abbotsford BC  US – Marshall WI, Meansville GA, Panama City FL

Two non-treated checks were included in the experiment, and ALTOSID XR-Briquettes (active ingredient: (S)Methoprene) were also tested as a positive control. ALTOSID XR-Briquettes (EPA Registration No.: 2724-421) were purchased from Central Life Sciences.

Mosquiron 2010 Field Trials

Experimental Design Table 1. Products and Rates Tested Treatment Number

Treatment Name

Amount/ Type of Formulation

Minimum Amount of Water (L) added

1

Control-A

0

200

Type of Organic Matter *

Mosquito food only Sod grass & 2 Control-B 0 200 mosquito food Sod grass & 3 Positive Control 1 Altosid XR Briquette 200 mosquito food Sod grass & 4 Pastille 0.5X 20 grams of pastilles 200 mosquito food Sod grass & 5 Pastille 1X 40 grams of pastilles 200 mosquito food Sod grass & 6 Pastille 2X 80 grams of pastilles 200 mosquito food 7 CRD 0.5X ½ CRD** 200 Mosquito food only 8 CRD 1X 1 CRD 200 Mosquito food only 9 CRD 2X 2 CRDs 200 Mosquito food only * The FL-1 site utilized existing concrete tanks with soil and vegetation established in the bottom of the tanks for Treatments 2 through 6. ** CRDs were cut in half for the 0.5X rate.

Galvanized metal tanks (approximate capacity of 250 liters) were used at all sites except FL-1. The FL-1 site utilized permanent concrete tanks. For treatments 2 through 6, a

Mosquiron Table 3. Mosquito Egg Sources and2010 Genera

Field Trials

Trial No.

Egg Source

Genera

BC-1

From wild mosquitoes laying eggs in small artificial pools.

Culex spp.

FL-1

Reared and harvested by Ken Shaffer at the Public Health Entomology Research and Education Center, Panama City, FL

Culex quinquefasciatus

GA-1

Reared and harvested by Steve Sackett, New Orleans Mosquito and Termite Control Board (NOMTCB).

Aedes aegypti

MB-1

From wild mosquitoes laying eggs in small artificial pools.

Culex spp.

WI-1

From wild mosquitoes laying eggs in small artificial pools.

Culex restuans and Culex pipiens

Mosquiron 2010 Field Trials Experimental Design  Eggs were introduced to treatment tanks approx . every 2 weeks  Culex eggrafts were placed directly on water surface at WI, MB, BC and

FL sites  Once hatching occurred all trts. received a larval liquid food slurry

 Food slurry was a standard formula consisting of liver powder/brewer’s

yeast  Food administered 3-4 times/wk. until water was cloudy in appearance

Mosquiron 2010 Field Trials Marshall - WI site

Meansville - GA site

Mosquiron 2010 Field Trials Panama City -FL Site

Mosquiron 2010 Field Trials Portage la Prairie - MB Site

Mosquiron 2010 Field Trials Abbotsford - BC Site

Mosquiron 2010 Field Trials Data Collection  Treatments were inspected for the presence of live larvae and/or pupae

on an as needed basis. Once 3rd and/or 4th instars were present, dips were taken to harvest live larvae.  Florida A&M’s PHEREC Standard Mosquito Sampling Method was used

at all trial sites.  Using a standard mosquito dipper, eight dips were taken per treatment.  The number of larvae and/or pupae per dip were counted and

recorded. Larvae were returned to the treatment tanks.  Once pupae were found, they were transferred to emergence jars for

monitoring.  Adults that emerged successfully from pupal cases were counted and

recorded as successful hatches. Dead adult mosquitoes on the water surface recorded as unsuccessful emergence.

Observations  Using local sources for harvesting Culex eggrafts delayed start of trial in

Northerly sites to early July  Was exacerbated by a cool wet spring in Canada and WI  FL location received very high precipitation (18” rain in July) causing

significant dilution of novaluron in cement troughs

Conclusions  Study results demonstrate Mosquiron formulations provided 100% IE

for duration of study at all locations  100% IE observed at FL site with 2x CRD