Ppt Slide

January 29, 2018 | Author: Anonymous | Category: Science, Health Science, Urology
Share Embed Donate


Short Description

Download Ppt Slide...

Description

USE OF P63 IN ASSESSING MIMICS OF PROSTATIC ADENOCARCINOMA DR. A. T. ATANDA, BAYERO UNIVERSITY/AMINU KANO TEACHING HOSP. KANO

BACKGROUND Prostatic carcinoma has several well documented mimics, particularly on core needle biopsy, for which the pathologist cannot rely on morphology alone for their differential diagnosis.  Correct evaluation of these mimics will help prevent unnecessary treatment for cancer. 

Mimics of Prostatic Carcinoma

Atrophy Basal Cell Hyperplasia Adenosis Seminal vesicles Ganglia Paraganglia 

AIM

To evaluate the reliability of morphology alone measured against immunohistochemistry in evaluating equivocal prostatic biopsies.



METHODOLOGY p63 immunohistochemical stain (clone BC4A4)  equivocal impressions of benign versus malignant diagnoses over a  12-month period (Jan – Dec., 2013)  Negative stain = positive for malignancy  Positive stain = negative for malignancy 

Criteria for diagnosis of CaP Major criteria  infiltrative acini which may have cribriform disposition  absence of basal cells; and  nuclear and/or nucleolar enlargement. Minor criteria included: adjacent high grade PIN and nuclear hyperchromasia  others  mucinous fibroplasia, perineural invasion and glomerulations

Results 61 cases of CaP  27(44.3%) were considered equivocal.  6 (22.2%) of the 27 showed basal cell staining  3 cases of atrophy  basal cell hyperplasia  clear cell cribriform hyperplasia  Urothelium 

Results The probability of erroneous interpretation ranged btw 8.7% and 42.3%



PPV of morphology alone was 77.8%.



RESULT ctd 





Sensitivity of 100% (83.8% to 100%); Specificity of 77.8% (57.7% to 91.3%); NPV of 100%.

Discussion Our study  22.2% reclassified; Error rate 8.7% (sensitivity:77.8%; specificity: 100%)  7.5% error rate reported by Berney et at1 (London)  1.3% documented by Epstein et al2 in the US  100% and 64%.3 

Reasons for lower specificity by morphology Surgeons Sample adequacy (volume & number) Crush artifacts Cauterization artifacts  Pathologists Good sections inexperience 

Discussion 

The use of immunohistochemical staining for p63

protein alone, or as a component of a cocktail comprising

p63,

alpha-methyl-CoA-racemase

(AMACR) and Cytokeratin CK903,3 is also gaining momentum.

conclusion 

For improvement in diagnosis of carcinoma of prostate, particularly from core needle biopsies, there is need for pathologists to pay attention to the common mimics of carcinoma and incorporate immunohistochemistry into their diagnostic armamentarium.

References 1. Berney DM, Fisher G, Kattan MW, Oliver RT, Møller H, Fearn P, et al; Trans-Atlantic prostate group. Pitfalls in the diagnosis of prostatic cancer: retrospective review of 1791 cases

z

with clinical outcome. Histopathology 2007; 51: 452-7.

2. Epstein JI, Walsh PC, Sanfilippo F. Clinical and cost impact of second-opinion pathology: review of prostate biopsies prior to radical prostatectomy. Am J Surg Pathol 1996; 20: 851-7. 3. Tokumaru Y, Harden S V, Sun D I, Yamashita K, Epstein J I, Sidransky D. Optimal use of a

panel of methylation markers with GSTP1 hypermethylation in the diagnosis of prostate adenocarcinoma. Clin Cancer Res 2004; 10: 5518-22.

View more...

Comments

Copyright � 2017 NANOPDF Inc.
SUPPORT NANOPDF