CURRICULUM VITAE
Vana Spoulou, MD, MPhil, PhD Associate Professor of Paediatric Infectious Diseases Date of Birth: 26 Feb 1962 Nationality: Greek Address: "Agia Sophia" Children's Hospital Infectious Diseases Dept. Thivon and M. Asias 1, GOUDI 11 527 Athens - GREECE Tel.: 0030 210 74.67.673 Fax: 0030 210 77.95.762 E-mail:
[email protected]
GRADUATE EDUCATION 1979-1985 Medical student, School of Medicine, National and Kapodistrian University of Athens POST GRADUATE EDUCATION 1991-1995 MPhil student, Institute of Child Health, UCL London, UK PROFESSIONAL TRAINING June 1987-Oct 1991 Senior House Officer in Paediatrics Jan 1992-March 1995 Clinical fellow in Infectious Diseases Great Ormond Street Hospital for Sick Children London UK MPhil Student Immunobiology Institute of Child Health University College London QUALIFICATIONS July 1985 25/11/1991 30/09/1996 4/07/1997 10/10/1998
MD School of Medicine, National and Kapodistrian University of Athens Membership in Paediatrics Clinical Infectious Diseases Specialist Doctor of Philosophy, Athens University Medical School PhD THESIS in Epidemiology: Accidents in the first 7 years of Life Master of Philosophy in Immunobiology, UCL, London, UK. MPhil THESIS: Quantitative and qualitative aspects of immune responses to polysaccharide vaccines in HIV-1 infected children 2002-ADVAC Advanced Course in Vaccinology Annecy-France (Prof Claire-Anne Siegrist)
CURRENT APPOINTMENT Assistant Professor in Paediatric Infectious Diseases, 1st Dept of Paediatrics, School of Medicine, National and Kapodistrian University of Athens, “Agia Sophia” Children’s Hospital, Athens (Prof G. Chrousos). Specialist Registrar in Paediatric Infectious Diseases at “Aghia Sophia” Children’s Hospital Infectious Diseases Dept MAKKA. I am responsible for all Greek HIV-infected children referred to our HIV outpatient clinic. My clinical responsibilities are also relevant to vaccine-preventable diseases since my department serves as a reference unit for treating patients with vaccinepreventable disases e.g during epidemics of measles, influenza, meningitis etc. I have also organised a vaccine outpatient clinic where different high risk patients referred to us by haematology-oncology, BMT unit, congenital haemoglobulinopathies unit etc for advise on their vaccination coverage. Head of the Immunobiology Research Lab at “Aghia Sophia” Children’s Hospital, involved in bacterial carbohydrate and protein vaccine research. I am actively involved in vaccinology since 1992 when as an MPhil student at the Institute of Child Health, UCL, UK, I developed serological methods for the investigation of " Quantitative and qualitative aspects of immune responses to polysaccharide vaccines among HIV-1 infected children" (MPhil Thesis; UCL, 1998). Following the completion of my studies in UK, I returned to Greece, where I established and run the Immunobiology research lab at "Agia Sophia" Children's Hospital, in Athens. Research in the laboratory focuses on the investigation of naturally and vaccine-induced immunity to a number of bacterial pathogens including S pneumoniae, Haemophilus influenzae type b (Hib) and Neisseria meningitides. ELISA for the measurement of antibodies to Hib, N. Meningitidis and S pneumoniae are routinely run in the laboratory in conjunction with clinical trials. We are interested in the determination of protective vaccine-induced immunity in normal and highrisk groups. For that purpose we have developed a number of different immunological methods for identification of immunological correlates of protection e.g measurement of antibody avidity, specificity, Ig isotypes, opsonophagocytic and bactericidal antibody activity. An important focus of my research is the investigation of vaccine-induced immunological memory by a range of different methods e.g by FACS, cytokine production by ELISA and ELISPOT and antigen-induced proliferation assays which have been accordingly modified for early detection of specific memory cells. Methods developed in my lab have been applied in the investigation of vaccine–induced protective immunity in children with HIV, in premature babies, children with malignancies, bone marrow transplants and patients with Congenital Haemoglobulinopathies. In 2000 I was awarded with the Young Investigator Award by the European Society of Paediatric Infectious Diseases for my research on the ‘Kinetics of the immune response to the 23valent polysaccharide vaccine in bone- marrow transplant reciepients.’ In 2010 my research on the .The effect of anti-TNF treatment on the immunogenicity and safety of the 7-valent conjugate pneumococcal vaccine in children with juvenile idiopathic arthritis. published in the Vaccine. 2010 Jul 12;28(31):5109-13 was awarded with the 1st Award of Greek Society of Paediatrics In 2011 my research group was awarded with the 2nd Award of the Greek Medical Society for our work on the Identification of immunodominat B cell epitopes within pneumococcal surface proteinS. Since 2008 I am member of the European rotavirus vaccine.Expert Group. I am member of the editorial Board of BMC Pediatrics and I serve as reviewer in many journals including Vaccine.
PUBLICATIONS
Autoimmune hepatitis type-2 and Epstein-Barr virus infection in a toddler: art of facts or an artifact? Zellos A, Spoulou V, Roma-Giannikou E, Karentzou O, Dalekos GN, Theodoridou M. Ann Hepatol. 2013 Jan-Feb;12(1):147-51. Antibiotic therapy for pediatric community-acquired pneumonia: do we know when, what and for how long to treat? Esposito S, Cohen R, Domingo JD, Pecurariu OF, Greenberg D, Heininger U, Knuf M, Lutsar I, Principi N, Rodrigues F, Sharland M, Spoulou V, Syrogiannopoulos GA, Usonis V, Vergison A, Schaad UB. Pediatr Infect Dis J. 2012 Jun;31(6):e78-85. Treatment-associated polymorphisms in protease are significantly associated with higher viral load and lower CD4 count in newly diagnosed drug-naive HIV-1 infected patients. Theys K, Deforche K, Vercauteren J, Libin P, van de Vijver DA, Albert J, Asjö B, Balotta C, Bruckova M, Camacho RJ, Clotet B, Coughlan S, Grossman Z, Hamouda O, Horban A, Korn K, Kostrikis LG, Kücherer C, Nielsen C, Paraskevis D, Poljak M, Puchhammer-Stockl E, Riva C, Ruiz L, Liitsola K, Schmit JC, Schuurman R, Sönnerborg A, Stanekova D, Stanojevic M, Struck D, Van Laethem K, Wensing AM, Boucher CA, Vandamme AM; SPREAD-programme. Retrovirology. 2012 Oct 3;9:81. Complicated Pneumococcal Meningitis in a Fully Vaccinated Child: Value of Magnetic Resonance Imaging Monitoring. S Ilia , A.- M. Spanaki , M. Raissaki , V. I. Spoulou , M.- D. Fitrolaki1 , M. Kanariou , G. Tzanakaki, G. Briassoulis Neuropediatrics 2011; 42: 240–244 Safety, immunogenicity and kinetics of immune response to 7-valent pneumococcal conjugate vaccine in children with idiopathic nephrotic syndrome. Liakou CD, Askiti V, Mitsioni A, Stefanidis CJ, Theodoridou MC, Spoulou VI. Vaccine. 2011 Jul 2 Summary of effectiveness and impact of rotavirus vaccination with the oral pentavalent rotavirus vaccine: A systematic review of the experience in industrialized countries. Giaquinto C, Dominiak-Felden G, Van Damme P, Htar Myint TT, Maldonado YA, Spoulou V, Mast TC, Staat MA. Hum Vaccin. 2011 Jul 1;7(7). Monitoring of lipodystrophic and metabolic abnormalities in HIV-1 infected children on antiretroviral therapy. Spoulou V, Kanaka-Gantenbein C, Bathrellou I, Mora S, Mostrou G, Sidossis L, Chrousos G, Theodoridou M. Hormones (Athens). 2011 Apr;10(2):149-55. Natural and vaccine-induced immunity to Neisseria meningitidis serogroup C in asplenic patients with β-thalassemia. Spoulou V, Tzanakaki G, Lekka S, Chouliaras G, Ladis V, Theodoridou M. Vaccine. 2011 Jun 15;29(27):4435-8. Cognitive and psychosocial development of HIV pediatric patients receiving highly active antiretroviral therapy: a case-control study. Thomaidis L, Bertou G, Critselis E, Spoulou V, Kafetzis DA, Theodoridou M. BMC Pediatr. 2010 Dec 27;10:99. A 2-year-old girl with fever, abdominal pain, and lung cavities. Vasilopoulou V, Makris A, Mostrou G, Spoulou V, Syriopoulou V, Theodoridou M. Pediatr Infect Dis J. 2010 Nov;29(11):1061, 1065-6. No abstract available.
The effect of anti-TNF treatment on the immunogenicity and safety of the 7-valent conjugate pneumococcal vaccine in children with juvenile idiopathic arthritis. Farmaki E, Kanakoudi-Tsakalidou F, Spoulou V, Trachana M, Pratsidou-Gertsi P, Tritsoni M, Theodoridou M. Vaccine. 2010 Jul 12;28(31):5109-13. Epub 2010 May 12. Vaccine-associated herpes zoster ophthalmicus [correction of opthalmicus] and encephalitis in an immunocompetent child. Chouliaras G, Spoulou V, Quinlivan M, Breuer J, Theodoridou M. Pediatrics. 2010 Apr;125(4):e969-72. Ascariasis in an adolescent with AIDS. Roma-Giannikou E, Viazis N, Spoulou V, Theodoridou M. J Pediatr Gastroenterol Nutr. 2009 Jan;48(1):1. The effect of 23-valent pneumococcal polysaccharide vaccine on immunological priming induced by 7-valent conjugate vaccine in asplenic subjects with beta-thalassemia. Orthopoulos GV, Theodoridou MC, Ladis VA, Tsousis DK, Spoulou VI. Vaccine. 2009 Jan 14;27(3):350-4. Objective analysis of retinal function in HIV-positive children without retinitis using optical coherence tomography. Moschos MM, Mostrou G, Psimenidou E, Spoulou V, Theodoridou M. Ocul Immunol Inflamm. 2007 Jul-Aug;15(4):319-23. Conjugate vaccine-induced immunological priming is meningococcal C infection. Spoulou VI, Moschou CK, Tzanakaki G, Theodoridou MC. Vaccine. 2007 Oct 10;25(41):7012-3.
not
protective
against
acute
Natural and vaccine-induced immunity against Haemophilus influenzae type b in patients with beta-thalassemia. Spoulou VI, Tsoumas DL, Ladis V, Spentzou A, Theodoridou MC. Vaccine. 2006 Apr 12;24(16):3050-3. Immunogenicity and immunological memory induced by a 7-valent pneumococcal CRM197 conjugate vaccine in symptomatic HIV-1 infected children. Spoulou VI, Tsoumas DL, Papaevangelou VG, Mostrou GI, Theodoridou MC. Vaccine. 2005 Nov 16;23(46-47):5289-93. Long-term immunity to measles, mumps and rubella after MMR vaccination among children with bone marrow transplants. Spoulou V, Giannaki M, Vounatsou M, Bakoula C, Grafakos S. Bone Marrow Transplant. 2004 Jun;33(12):1187-90. Haemophilus influenzae type b conjugate vaccine-induced immunological memory in symptomatic HIV-1-infected children. Spoulou VI, Tsoumas DL, Papaevangelou VG, Mostrou GI, Theodoridou MC. AIDS. 2003 Jun 13;17(9):1396-8. Protein conjugate pneumococcal vaccines. Spoulou V, Gilks CF, Ioannidis JP. BMJ. 2002 Mar 30;324(7340):750-1. No abstract available. Erratum in: BMJ 2002 Apr 27;324(7344):1002. 23-valent pneumococcal vaccination and HIV. Spoulou V, Theodoridou M, Papaevangelou VG, Mostrou GI, Ioannidis JP. Lancet. 2000 Sep 16;356(9234):1027-8.
Kinetics of antibody concentration and avidity for the assessment of immune response to pneumococcal vaccine among children with bone marrow transplants. Spoulou V, Victoratos P, Ioannidis JP, Grafakos S. J Infect Dis. 2000 Sep;182(3):965-9. Persistence of antibody responses to Haemophilus influenzae type b polysaccharide conjugate vaccine in children with vertically acquired human immunodeficiency virus infection. Gibb D, Giacomelli A, Masters J, Spoulou V, Ruga E, Griffiths H, Kroll S, Giaquinto C, Goldblatt D. Pediatr Infect Dis J. 1996 Dec;15(12):1097-101. Hypogammaglobulinaemia secondary to reflux oesophagitis. Spoulou V, Melville C, Young M, Milla P, Newman C, Morgan G. Arch Dis Child. 1995 Mar;72(3):245-6. Antibody responses to Haemophilus influenzae type b and Streptococcus pneumoniae vaccines in children with human immunodeficiency virus infection. Gibb D, Spoülou V, Giacomelli A, Griffiths H, Masters J, Misbah S, Nokes L, Pagliaro A, Giaquinto C, Kroll S, et al. Pediatr Infect Dis J. 1995 Feb;14(2):129-35.
PRIZES AND AWARDS 1992: HOREMEIO AWARD (1st PRIZE) awarded from the Hellenic Paediatric Association 1997: PREVENTIVE MEDICINE AWARD awarded from the Hellenic Paediatric Association 2001: European Society for Paediatric Infectious Diseases (ESPID) ΥOUNG INVESTIGATOR AWARD MEMBER of 1. Greek Medical Association 2. Hellenic Society of Infectious Diseases 3. Hellenic Society of Paediatric Infectious Disease 4. European Society of Paediatric Infectious Disease (ESPID)
INVITED TALKS ‘Clinical effectiveness of polysaccharide vaccines in high risk patients’, University of Ioannina, April 2001, Ioannina ‘The protective immunity of conjugated polysaccharide vaccines’, University of Thessaloniki’, May 2001, Thessaloniki ‘New antibiotics’, Seminar on Pediatric Infections, September 2001, Thessaloniki ‘Advantages in streptococcal infections’, 37th Annual Meeting of First Department of Pediatrics, University of Athens, April 2004, Athens ‘News on streptococcal infections’, Seminar of Pediatric Association of Cyprus, May 2004, Leukosia ‘Vaccines and Vaccinations: Theory and practice’, Seminar of 2nd Department of Pediatrics, University of Athens, March 2007, Athens ‘Vaccines for the child- traveler’, 40th Annual Meeting of Pediatrics, April 2007, Athens ‘Management of the child with fever and rush’, 5th cycle of continuous education in General Medicine, March 2008, Athens ‘Vaccines: Novelties and Prospects’, 41st Annual Meeting for Pediatrics, May 2008, Athens ‘HPV Infections in Infants and Children’, 42nd Annual Meeting for Pediatrics, April 2009, Athens ‘Rotavirus vaccination in Greece’, European Expert Meeting, Rotavirus Vaccination, May 2009, Finland ‘The anti-viral drugs in Pediatrics’, 13th Scientific Meeting for Pediatric Infections, October 2009, Athens ‘General principles of Vaccinations’, Postgraduate Seminar of Pediatric Infections, November 2009, Athens
‘Antiviral agents in Paediatrics’, 1st East-European and Mediterrannean Teaching Course in Pediatrics, December 2009, Brasov ‘Old and New Pneumococcal Vaccines’, 43rd Annual Meeting for Pediatrics, April 2010, Athens ‘Vaccines against Rota-virus’, Vaccines 2010, June 2010, Crete ‘Immunogenetics in the diagnosis of infections’, 14th Scientific Meeting for Pediatric Infections, October 2010, Athens ‘Vaccination strategies in immunocompromised patients’, 2nd East-European and Mediterranean Teaching Course on Pediatric Infectious Diseases, November 2010, Brasov ‘The Greek experience’, 2nd European expert Meeting on Rotavirus Vaccination, April 2011, Padova ‘The benefits of a Rotavirus vaccine’, Annual Meeting of European Society of Pediatric Infectious Diseases, June 2011, The Hague ‘Vaccine policy in Greece’, 1st Joint Conference of the Hellenic College of Pediatrics and The Royal Society of Medicine, May 2011, Athens
