Online Resource 3 Dynamics of the endosymbiont Rickettsia in an insect pest Microbial Ecology Bodil N. Cass1, Rachel Yallouz2, Elizabeth C. Bondy3, Netta Mozes-Daube2, A. Rami Horowitz4, Suzanne E. Kelly3, Einat Zchori-Fein2, Martha S. Hunter3§ 1
Graduate Interdisciplinary Program in Entomology and Insect Science, 410 Forbes, University of Arizona, University of Arizona, Tucson, AZ 85721 USA 2 Department of Entomology, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay, Israel 3 Department of Entomology, 410 Forbes, University of Arizona, Tucson, AZ 85721 USA 4 Department of Entomology, ARO, Gilat Research Center Mobile Post Negev, 85280, and Katif Research Center, Sedot Negev, Israel §
Corresponding author,
[email protected]
Fig. S1 Phylogenetic analysis of citrate synthase (gltA) gene sequences using maximum likelihood places the Israel field, Israel laboratory, USA field and USA laboratory sequences in the same clade along with other Rickettsia from B. tabaci. Bootstrap probability values ≥75 are indicated above branches, whereas posterior probabilities ≥0.95 of the topologically identical BI consensus tree are below branches. Sequences from this study are indicated in bold. Sequences from GenBank show accession numbers in parentheses. Rickettsia groups are indicated in upper case text. Rickettsia endosymbionts without species names are indicated with (s) and the host name.
Fig. S2 Phylogenetic analysis of rpmE-tRNAfMet intergenic spacer sequences using maximum likelihood places the Israel field, Israel laboratory, USA field and USA laboratory sequences in the same clade along with other Rickettsia from B. tabaci. Bootstrap probability values ≥75 are indicated above branches, whereas posterior probabilities ≥0.95 of the topologically identical BI consensus tree are below branches. Sequences from this study are indicated in bold. Sequences from GenBank show accession numbers in parentheses. Whilst this tree shows clearly that this fast-evolving sequence is invariant among USA and Israel Rickettsia, there was no statistical support for the other clades in the tree, indicating that this region that is very useful to discriminate bacterial species and strains, is likely not suitable for phylogenetic reconstruction, perhaps as it is evolving too quickly.
